For this function, a 1334 bp cDNA fragment masking nucleotides eighty one,414 of the mouse FgfrL1 mRNA (AJ293947) [fifteen] corresponding to amino acids one-440 was inserted in-frame into the EcoRI-BamHI web-site of the GFP expression vector pEGFP-N3 (Clontech)
We therefore attempted to elucidate the mechanism, by which FgfrL1 may take part in signaling. When we aligned the intracellular sequences of FgfrL1 from diverse vertebrates in a multiple sequence…