Ed.C2014 The Authors. Regeneration published by John Wiley Sons Ltd.P. Wu et al.Signaling Molecules in Lizard Scale RegenerationMicroscopic methodsAdult samples had been fixed in 4 paraformaldehyde in 0.1 mol/L phosphate buffer overnight and decalcified with 0.five mol/L ethylenediaminetetraacetic acid for 3 days to 1 week at 4 C. Seven micron paraffin sections had been ready. Sections had been treated for antigen retrieval with 0.01 mol/L citrate buffer (pH six.0) by microwaving for 6 min. Immunohistochemistry was performed according to Jiang et al. (1998) working with the following antibodies (1:200 dilution): -catenin (Sigma; C2206, St Louis, MO); PCNA (Chemicon; CBL407, Billerica, MA); NCAM and tenascinC (Chuong and Chen 1991). Secondary antibodies have been either biotinylated anti-mouse IgG or anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA; 1:200 dilution). The tertiary antibody was streptavidin (Vector Laboratories, 1:200 dilution). An AEC substrate kit (Vector Laboratories) was utilised to develop the staining. Hematoxylin was utilised to perform faint counterstaining. BrdU staining was performed based on Wu et al. (2004). For BrdU (BD; 347580; 1:200 dilution)/-catenin or PCNA/-catenin double staining, secondary antibodies Alexa Fluor antirabbit-488 (A11008) and anti-mouse-546 (A11030) from Invitrogen (Grand Island, NY) have been utilized at a 1:200 dilution. 4′,6-Diamidino-2-phenylindole (DAPI) was applied to visualize the nuclei. Stained sections have been imaged with a Zeiss 510 confocal microscope.AcknowledgmentsThis study was supported by the NIAMS through grants AR 42177, 47364 (to CMC) and was in aspect assistance by a University of Bologna Grant (60 ) (to LA). Confocal microscopy was performed by the Cell and Tissue Imaging Core from the USC Study Center for Liver Ailments (NIH Grant No. P30 DK048522 and S10 RR022508).
ArticleGestational palmitic acid suppresses embryonic GATA-binding protein four signaling and causes congenital heart diseaseGraphical abstract AuthorsRui Zhao, Li Cao, Wen-Jun Gu, …, Jing Cao, Kun Sun, Jian-Yuan ZhaoCorrespondencehusq04@163 (X.-Y.Z.), caojing73@126 (J.C.), [email protected] (K.S.), [email protected] (J.AAA -Y.Z.)In briefZhao et al. report that elevated maternal serum palmitic acid (PA) is associated with all the danger of congenital heart disease (CHD) in offspring. PA induces CHD phenotypes in mice through advertising K-Hcy of GATA4, therefore inhibiting its transcriptional activity.IL-6 Protein, Human Inhibition of K-Hcy rescued PA-induced CHD occurrence.PMID:23715856 HighlightsdMaternal higher palmitic acid is related with improved risk of CHD in offspring Palmitic acid promotes MARS and K-Hcy modification by means of activating NF-kB GATA4 transcriptional acitivity is inhibited by K-Hcy modification Inhibition of K-Hcy rescued PA-induced CHD occurrence in micedddZhao et al., 2023, Cell Reports Medicine four, 100953 March 21, 2023 2023 The Author(s). https://doi.org/10.1016/j.xcrm.2023.llllOPEN ACCESSArticleGestational palmitic acid suppresses embryonic GATA-binding protein four signaling and causes congenital heart diseaseRui Zhao,1,7 Li Cao,two,7 Wen-Jun Gu,two,7 Lei Li,3,7 Zhong-Zhong Chen,four,7 Jie Xiang,2 Ze-Yu Zhou,two Bo Xu,five Wei-Dong Zang,3 Xiang-Yu Zhou,2,* Jing Cao,3,* Kun Sun,1,* and Jian-Yuan Zhao1,three,6,8,*1Institute for Developmental and Regenerative Cardiovascular Medicine, MOE-Shanghai Essential Laboratory of Children’s Environmental Wellness, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China 2Obstetrics Gynecology Hospital of Fudan Unive.