Odies in relevant in vitro models. Coupling immune-isolation to pre-analytical protocols for urine processing and sample high-quality testing enables detection of a panel of endometrial genes in urine-recovered EVs. Summary/Conclusion: Overall, the study supplies a tool for non-invasive monitoring from the functional status of the endometrium, supporting biomedical niches which include assisted fertilization and diagnosis of endometriosis. Funding: ENDEvor POR Region Tuscany (identification on the project) and Exosomics R D ProgrammePF09.CD178/FasL Proteins custom synthesis Unveiling autologous blood doping: comparative evaluation of various purification tactics for urinary extracellular vesicles pioneering miRNA biomarker research Veronika Mussacka, Georg Wittmannb and Michael Pfafflc TUM College of Life Sciences Weihenstephan, Division of Animal Physiology and Immunology, Freising, Germany, Freising, Germany; bDepartment for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany, M chen, Germany; cAnimal Physiology and Immunology, College of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany, Freising, Germanyafive different isolation strategies have been evaluated: ultracentrifugation, membrane affinity, spin column chromatography, immunoaffinity and precipitation. After EV characterization by nanoparticle tracking analysis, western blotting, and transmission electron microscopy, total RNA was isolated as well as a library for Tissue Factor/CD142 Proteins Biological Activity little RNA sequencing was ready. The resultant thriving tactic was then applied to each of the collected samples which were equally analysed concerning their EV distribution and miRNA content material. Benefits: The comparative analysis disclosed enormous discrepancies with respect to EV yield, population, and purity, too as RNA yield and detected miRNAs. By applying the most beneficial performing tactic, which was based on immunoaffinity, drastically greater amounts of urinary EVs and many considerably differentially regulated miRNAs were observed just after ABD. Summary/Conclusion: Urinary EVs and their miRNA profile hold certainly promising attempts for the clear separation of ABD and non-doped athletes. Furthermore, the incorporated complicated comparative methodological analysis contributes enormously to future standardization and comparability of urinary EV research. Funding: The existing project has been financially supported by the WADA.PF09.Extracellular vesicles as graft biomarkers to address lung transplantation outcome Mario Barilania, Ilaria Righib, Giuseppe Buonoc, Lorenzo Rossod, Mario Nosottie and Lorenza Lazzarica Unit of Regenerative Medicine Cell Factory, Department of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy; Universitdegli Studi di Milano, Milan, Italy; bThoracic Surgery and Lung Transplant Unit, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; cUnit of Regenerative Medicine Cell Factory, Division of Transfusional Medicine and Hematology, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milano (MI), Italy, Milano, Italy; dUniversitdegli Studi di Milano, Milano (MI), Italy, Milano, ItalyIntroduction: Autologous blood doping (ABD) increases the oxygen capacity through re-infusion of a person’s autologous red blood cells. It is consequently employed by endurance athletes with a high amount of unreported instances, specially because reputable methods for unequivocal detection are nevertheless lacking. To su.