Cultured order-level divisions phylogenetically distant from known isolates. Members from the order S085 have been discovered in the surface sediments, although GIF3, GIF9, Napoli-4B-65, and MSBL5 had been prevalent within the deep layers. The phylum Verrucomicrobia was mainly represented by the household Rubritaleaceae (Rogaratinib Protein Tyrosine Kinase/RTK genera Haloferula, Luteolibacter, Persicirhabdus, Roseibacillus, and Rubritalea) discovered in the surface sediments (as much as eight). A comparable distribution was observed for Actinobacteria (largely on the order Actinomarinales), whose share reached 18.7 . Members of Planctomycetes (Phycisphaerales), Nitrospirota (Nitrospira sp.), Myxococcota, Firmicutes, Bacteroidetes (Cyclobacteriaceae), Gemmatimonadota, Patescibacteria, Latescibacterota, subgroups 21 and 22 of Acidobacteria, and candidate division NB1-j had been also identified preferentially inside the upper sediments, even though members of Acetothermia and Spirochaetota had been predominant within the deep layers. Bacteria of the phylum Desulfobacterota (delta-proteobacteria in standard taxonomy) were abundant in the upper sediments at station 6844 and in all sediment samples collected at station 6841, however they were identified in minor amounts in upper sediments at stations 6840, 6849, and 6864. Interestingly, in the upper sediments at stations 6841 and 6844, most of Desulfobacterota belonged for the households Desulfobulbaceae, Desulfocapsaceae, and Sva1033 group, even though within the deep sediments (station 6841, six cm and 169 cm layers) members of Desulfatiglandaceae (Desulfatiglans sp.), Desulfosarcinaceae, Dissulfuribacteraceae (SEEP-SRB2 group), and Syntrophobacterales prevailed. Various bacterial lineages were identified preferentially in deep sediments and were nearly absent in the surface sediments samples. Probably the most abundant was the phylum Caldatribacteriota (JS1 lineage), representing 4.two and 9.9 of the microbiome in the 6 cm and 169 cm horizons, respectively. Sulfurovum sp. (phylum Campilobacterota) and members in the phylum Calditrichota had been also located mostly within the deep sediments, and their shares enhanced with depth. Aminicenantes (recognized as class Aminicenantia within the phylum Acidobacteria in the GTDB taxonomy) were identified exclusively inside the deep sediments, exactly where they accounted for three of 16S rRNA sequences. As well as the key bacterial lineages, 36 other phylum-level divisions had been identified– namely, Aerophobota, Armatimonadota, Bdellovibrionota, Cloacimonadota, Cyanobacteria, Dadabacteria, Deferrisomatota, Deinococcota, Dependentiae, Elusimicrobiota, Entotheonellaeota, Fermentibacterota, Fusobacteriota, Fibrobacterota, Hydrogenedentes, Margulisbacteria, Methylomirabilota, Modulibacteria, Nitrospinota, Nitrospirota, Schekmanbacteria, Sumerlaeota, Zixibacteria, 10bav-F6, BHI80-139, CK-2C2-2, FCPU426, LCP-89, SAR406_clade, MBNT15, NKB15, RCP2-54, SAR324_clade, TA06, WOR-1, and WS2. Their shares had been less than 1 in all analyzed samples.Microorganisms 2021, 9,10 of3.3. Aerobic Methanotrophs Revealed by pmoA Gene Profiling To recognize (S)-3,4-DCPG Cancer methanotrophic bacterial lineages, we amplified and sequenced the libraries targeting the genes coding for any conserved area with the particulate methane monooxygenase subunit A (pmoA). The pmoA genes may be employed within the reconstruction of phylogenetic relationships among aerobic methanotrophs (reviewed in [49]). Taxonomic assignment of obtained OTUs showed that only two of them (OTU9 and OTU32) represented pmoA genes, and six OTUs belonged to evolutionary connected ammonia monooxygenase (amoA), which.