Product Name :
Anti-Halo Tag, Rabbit antibody
Applications:
WB,ELISA,IP
Reactivity :
Halo tag
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Halo Tag, Rabbit antibody is designed for detecting Halo tag fusion proteins. Anti-Halo Tag, Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-Halo Tag, Rabbit antibody detects the Halo tag selectively, no reactivity with other proteins. | | Immunogen: Halo-KLH | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc | Conjugate: Unconjugated | Specificity: Halo tag | Cross-Reactivity: No cross-reactivity with other proteins | Affinity: Dissociation constant KD of 1nM | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: PBS pH=7.{{1965291-08-0} medchemexpress|{1965291-08-0} Purity & Documentation|{1965291-08-0} Purity|{1965291-08-0} custom synthesis} 510mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: store at –20 °C. Avoid freeze / thaw cycle | Background:The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces.{{2819276-88-3} web|{2819276-88-3} Protocol|{2819276-88-3} References|{2819276-88-3} custom synthesis} Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, Rabbit antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.PMID:30252309
Description2 :
Anti-Halo Tag, Rabbit antibody is designed for detecting Halo tag fusion proteins. Anti-Halo Tag, Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-Halo Tag, Rabbit antibody detects the Halo tag selectively, no reactivity with other proteins.
Immunogen:
Halo-KLH
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Purity :
Recombinant Expression and Affinity purified
Buffer :
PBS pH=7.510mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage :
store at –20 °C. Avoid freeze / thaw cycle
Function:
WB: 1:5000-1:20,000 ELISA: 1:5,000-20,000ICC/IF: 1:200-1:1000IP: 1-2ug/sampleFlow Cyt： 1µg for 106 cellsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.