ine width with the edge indicates the strength on the data assistance. (B) The best 20 genes from the PPI network: X-axis represents degree, and Y-axis indicates genes.FIGURE 5 | Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of targets. (A) GO analysis of targets: X-axis represents GO terms, and Y-axis indicates the enrichment score [adjusted false discovery price (FDR)] in that term. (B) KEGG analysis of targets: Dot plot showed the top rated 20 KEGG ACAT1 medchemexpress pathways with adjusted FDR. The colour scale indicates the adjusted FDR, plus the dot size represents the gene count in every term.= 86, HQ15), baicalein (CYP26 Gene ID degree = 83, CQC2), stigmasterol (degree = 76, M), isorhamnetin (degree = 72, HQ5), dinatin (degree = 71, CQC1), jaranol (degree = 62, HQ2), 6-OH-luteolin(degree = 62, CQC5), and scutellarein (degree = 59, CQC9). Finally, we chose quercetin, luteolin, kaempferol, scutellarein, and stigmasterol to treat tension diarrhea in mice.Frontiers in Veterinary Science | frontiersin.orgOctober 2021 | Volume 8 | ArticleZhang et al.Anti-diarrhea Mechanism Evaluation of QJCTABLE two | Establishment of a mouse model of strain diarrhea. Group Typical control group Model control groupa,b SignificantThe accumulated quantity of stools 3.8 0.838a 11.six 1.141bThe variety of loose stools 0a 7.8 0.837bThe rates of loose stools ( ) 0a 0.68 0.074bdifferences at p 0.05.FIGURE 6 | Remedy of diarrhea mice with gradient dose of QJC. Data have been expressed because the mean SD (n = 5); the substantial difference between groups (p 0.05) was shown by the diverse letters above the histogram. (A) The accumulated number of stools. (B) The time of initial diarrhea. (C) The number of loose stools. (D) The rates of loose stools.Protein rotein Interaction Network AnalysisThe PPI network comprised 203 nodes and 595 edges (Figure 4A), which indicated 595 interactions. To clarify this additional, the prime 20 nodes have been extracted by the degree (Figure 4B). Within the interaction network, amyloid beta A4 protein (APP; degree = 34), phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1; degree = 27), Src protein-tyrosine kinase (SRC; degree = 26), B2 bradykinin receptor (B2R; degree = 24), and IL-8 (degree = 24) will be the top rated 5 prospective targets of QJC in the therapy of diarrhea, which may perhaps play a crucial part.involved in 44 enrichment outcomes, and also the cellular element (CC) was involved in 73 enrichment benefits. According to their enrichment score, we visualized the top rated ten pathways (Figure 5A). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was made use of to explore the possible mechanisms of QJC inside the therapy of pressure diarrhea. Consequently, 130 KEGG pathways were obtained, and also the best 20 KEGG signaling pathways were constructed according to FDR (Figure 5B).High-Performance Liquid Chromatographic ResultsQuercetin, kaempferol, luteolin, scutellarein, and stigmasterol were eluted, as shown in Supplementary Figure 1. Meanwhile, we calculated the content material of diverse elements within the sample using the regression equation. Quercetin, kaempferol, luteolin, scutellarein, and stigmasterol levels were 4.91 0.228, two.18 0.237, 25.58 0.543, 0.53 0.026, and 145.72 13.712 /g, respectively (Supplementary Table two).Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Enrichment AnalysesTo additional discover the biological traits on the 297 candidate targets of QJC, we performed GO evaluation on diarrhea targets. Consequently, the biological approach (BP) was involved in 292 enr