ted. Further file ten. Enriched terms in up-regulated genes involving ROCK1 supplier Supplemented Infected vs Manage Infected. Extra file 11. Enriched terms within the list of differentially upregulated genes amongst the groups Manage Infected vs Supplemented Infected. Extra file 12. Typical liveweight and age (typical deviation) for every group in the starting and end of your experiment. Additional file 13. Composition of the control protein and supplemented protein diets. Additional file 14. Bromatological composition (g kg-1 of dry matter at one hundred ) and power (MJ) of your diets’ ingredients. Added file 15. Bromatological evaluation of the diet. Additional file 16. List of ovarian RNA samples, their concentration, 260/280 and 260/230 absorbance ratios. More file 17. EM estimation algorithm procedure. Added file 18. Statistical methodology utilised in the Enrichment evaluation. Additional file 19. Protein Network analysis. Acknowledgements Not applicable. Arrive suggestions statement This study was carried out in compliance with the ARRIVE guidelines. Authors’ contributions P.S.H. performed the in vivo and laboratory experiments, analysed the information and wrote the manuscript. C.M.S.C. conducted the in vivo and laboratory experiments. R.C.L. performed the statistical analyses, assisted with manuscript writing and revision. D.G.C. helped with qPCR arranging and qPCR information analysis. L.M.K. cultivated and supplied the Haemonchus contortus larvae. S.M.T. assisted within the planning of experiments. H. L. elaborated the study design and style and planning, assisted inside the manuscript revision. The author(s) study and approved the final manuscript. Author’s information and facts Paula Suarez-Henriques is a veterinary surgeon with a Master in Animal Science as well as a Doctorate in Animal Science. She obtained both degrees inside the last six years in the University of S Paulo in Brazil. In July 2020, she presented part of her doctorate study in the Society for the Study of Reproduction PIM2 list virtual meeting. Ahead of that, she worked as a veterinarian clinician along with a analysis assistant in laboratories studying molecular pathology of breast cancers, cell and molecular biology in mammary gland development, and a number of myeloma resistance to alkylating agents. Funding We thank the National Council for Scientific and Technological Research -(CNPq – Brazil) and CAPES for the economic assistance with Paula SuarezHenriques’ PhD studentship bursary quantity 141846/2017 and, for funding the costs of reagents and gear important for the accomplishment of this investigation project, with approach numbers CNPq 311008/2016-0 and CNPq 420373/2016. We also thank S Paulo Research Assistance FoundationSupplementary InformationThe on the internet version consists of supplementary material obtainable at doi. org/10.1186/s12917-021-03020-7. More file 1: Table S1. RNA sequencing data high quality summary. Added file 2: Table S2. Number of sequences and Sequencing depth generated by RNA sequencing.Suarez-Henriques et al. BMC Veterinary Study(2021) 17:Page 22 of(FAPESP) method numbers 2017/50451 and 2017/18086-0, for extra funding for reagents. Availability of information and materials The datasets made use of and/or analysed for the duration of the current study are accessible inside the Extra files. The files which are not within this section may possibly be provided below affordable request to Dr. Helder Louvandini e-mail address: [email protected] approval This investigation project, with its protocol and procedures employed, had been eth