city against cancer cell lines; hepatocellular carcinoma HepG-2, cervical carcinoma HeLa, and the breast carcinoma cell lines, MCF-7 (Table five). Figure three represents the dose-response curve of the sage important oil batches around the viability of each of the cell lines applied in this study. As shown inside the curves, sage’s crucial oils ADAM17 Inhibitor custom synthesis inhibited all of the cancer cells, HepG-2, HeLa, and MCF-7, within a dose-dependent manner (one hundred /mL), though the curves displaying an upgrade pattern indicated the low cytotoxicity with the tested samples on the standard cells (MRC-5).Table five. IC50 and selective index values obtained for fresh and dried necessary oil batches of sage against different cancer and normal fibroblast lung (MRC-5) cell lines. Groups DOX FH 1WDH 2WDH 3WDH 4WDH MCF-7 IC50 SD 1.four 0.1 181.three 18.3 189.three 17.45 194.1 20.1 176.3 16.five 215.7 18.four SI 1.1 3.3 2.two two.six 2.three 1.9 HepG-2 IC50 SD 1.04 0.1 161.7 15.three 179.12 16.5 182.four 14.two 174.9 13.2 195.4 20 SI 1.5 3.7 2.4 two.7 two.three two.1 IC50 SD 1.02 0.1 142.1 12.four 164.1 15.five 174.7 13.6 127.5 11.4 176.2 18 HeLa SI 1.six four.two 2,6 2.9 3.2 2.4 MRC-5 IC50 SD 1.six 0.1 596.5 20 425.6 30.2 499.four 32 405.2 18.three 414.7 27.Molecules 2021, 26,16 ofFigure three. Dose-response curve of cytotoxicity of Adenosine A1 receptor (A1R) Inhibitor Gene ID doxorubicin as a positive handle (A), sage important oils (one hundred /mL) of FH (B), 1WDH (C), 2WDH (D), 3WDH (E), and 4WDH (F) on cancer cell lines breast (MCF-7), hepatic (HepG-2), and cervical (HeLa) in comparison to normal cells (MRC-5).Figure four shows the summarized comparisons between the IC50 values from the tested samples and the good manage, doxorubicin, around the cancer cell lines, and also the typical fibroblast cells. The 4WDH vital oil showed the lowest cytotoxicity on all of the tested cancer cell lines, i.e., HepG-2, HeLa, and MCF-7, with IC50 values ranging from 176.two /mL for HeLa cells to 215.7 /mL for the MCF-7 cell lines. The FH-based essential oil seemed to be obtaining the highest cytotoxic effects around the cancer cells, along with the lowest cytotoxic effects on the normal cells with all the highest SI: HeLa (IC50 = 142.1 /mL, SI = four.2), HepG-2 (IC50 = 161.7 /mL, SI = three.7), and MCF-7 cells (IC50 = 181.three, SI = three.three) (Table 5). A earlier study also demonstrated the capacity in the S. officinalis polar solvents extracted material to inhibit MCF-7 cancer cell lines with no significant cytotoxic effects against normal human umbilical vein endothelial cells with SI 3, and that is in agreement with our current findings [58].Figure 4. The cytotoxicity IC50 values in the sage vital oils ( /mL) on cancer cell lines breast (MCF-7), hepatic (HepG-2), and cervical (HeLa) in comparison towards the regular cells (MRC-5). Doxorubicin was made use of as a cytotoxic drug (positive control).Molecules 2021, 26,17 of4. Conclusions This report comprehensively demonstrated the extended drying impact of aerial parts of S. officinalis on oil yields, its top quality, and its effects on biological activities. Sage yielded the highest yields of necessary oil from the two-week shade and room-temperature dried plant supplies, with 0.28 of necessary oil procurement containing more than 99.82 0.18 from the sage oil’s reported componential constituents. The common chemotypic components were present in abundance, and presumably are a part of the active constituents with the critical oil against induced liver damages and active cancer cell lines, i.e., MCF-7, HepG-2, and HeLA. The herb and its vital oil exhibited effective properties and are advised for use in liver issues. Their prophylacti