PAMA1-PgpdA-cprA was generated as follows: PCR, applying the joint primers AMA1-gpdA-F/GpdA-cprA-F and GpdA-R/AMA1-BamHICprA-R, was made
PAMA1-PgpdA-cprA was generated as follows: PCR, applying the joint primers AMA1-gpdA-F/GpdA-cprA-F and GpdA-R/AMA1-BamHICprA-R, was made use of to produce gpdA promoter/cprA ORF. The two fragments had been fused collectively and…