Tients with diabetes. Approaches: Sufferers at Concord Hospital with suspected CAD gave written informed consent and were administered RIPC (sphygmomanometer on the arm, three five min cycles, n = 31) or sham (n = 29) prior to angiography, with recruitment ongoing. Blood was collected pre- and right away post-RIPC/sham and plateletfree plasma generated. Worldwide coagulation/fibrinolytic prospective was measured by all round haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and numerous fibrinolytic elements by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Study institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have potential as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying cause of heart attack and stroke, EV release could be dysregulated and their contents can mediate pro-inflammatory effects. Many markers have been previously identified on uEV including exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers consist of microRNAs (miRs). miR-21 and miR-155 are important regulatory miRs that are upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to possess pro-atherogenic effects and miR-155 deficiency in murine models results in lowered atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from sufferers diagnosed with coronary artery disease (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic individuals had been isolated via benchtop centrifugation. The concentration and size of uEVs had been MMP Species analysed through the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = 10 per group). uEV surface marker expression was analysed by Topo II drug ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Outcomes: uEV concentration in symptomatic patients (median; 6.46E+9 particles/mL) was substantially decreased (p 0.05) when compared with asymptomatic patients (median; 1.25E+10 particles/mL). CD11B+ uEVs had been enhanced and CD16+ uEVs had been decreased in the symptomatic individuals (p 0.01). Furthermore, the concentration of CD45+ EVs had been elevated in symptomatic patients (p 0.001). Though uEV miR-21 was unchanged, miR-155 expression was considerably enhanced within the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic prospective. As CAD severity increases, uEV concentration is lowered, surface marker expression is altered and uEV miR-155 expression is enhanced. Funding: The Irish Research Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Overall health Evaluative Sciences, Investigation Institute, The Hospital for Sick Children,.