Creased citations maximum visibility for your study: more than 100M web site views per yearAt BMC, analysis is generally in progress. Understand extra Adenosine A2B receptor (A2BR) Antagonist Source biomedcentral.com/submissions
Europe PMC Funders GroupAuthor Manuscript Endothelium. Author manuscript; out there in PMC 2006 March 13.Published in final edited form as: Endothelium. 2005 ; 12(5-6): 23341. doi:ten.1080/10623320500476559.Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsAtorvastatin Impacts Quite a few Angiogenic Mediators in Human Endothelial CellsJozef Dulak, Agnieszka Loboda, Agnieszka Jazwa, and Anna Zagorska Department of Health-related Biotechnology, Faculty of Biotechnology, Jagiellonian University, Krak , Poland Jacob D ler, Hannes Alber, Wolfgang Dichtl, Franz Weidinger, and SIRT2 Purity & Documentation Matthias Frick Division of Cardiology, Innsbruck University, Austria Alicja Jozkowicz Division of Health-related Biotechnology, Faculty of Biotechnology, Jagiellonian University, Krak , PolandAbstractThe pleiotropic effects of statins, inhibitors of 3-hydroxy-3-methylglutaryl oenzyme A (HMGCoA) reductase, have been lately extended for the modulation of angiogenesis. Right here, to acquire much more insight into the statins action, the authors have investigated the effect of atorvastatin on the expression of many angiogenic and inflammatory genes in human umbilical endothelial cells (HUVECs). Atorvastatin was proangiogenic at the dose of 10 nM, and antiangiogenic at the concentrations of 1 to ten M. Moreover, these higher concentrations inhibited also the proliferation of HUVECs induced by vascular endothelial growth aspect (VEGF). Reduce doses of atorvastatin did not influence endothelial cell proliferation. Importantly, atorvastatin in the micromolar concentrations diminished the production of interleukin (IL)-8, a proinflammatory and proangiogenic chemokine, and inhibited the synthesis of urokinase plasminogen activator (uPA), a potent proinflammatory mediator. However, it decreased also the expression of plasminogen activator inhibitor-1 (PAI-1) and thrombospondin-1 (TSP-1), the inhibitors of angiogenesis. Atorvastatin stimulated the expression of angiopoietin (Ang)-2 and moderately enhanced the expression of endothelial nitric oxide synthase (eNOS), whereas heme oxygenase-1 (HO-1) was not drastically impacted. In conclusion, the present findings points to other angiogenesis-related effects of atorvastatin, which might be of relevance towards the valuable influence of statins in cardiovascular method.Key phrases Atherosclerosis; Cancer; Heme Oxygenase-1; Interleukin eight; Vascular Endothelial Development Aspect Statins are potent inhibitors on the 3-hydroxy-3-methylglutaryl oenzyme A (HMG-CoA) reductase by way of blocking the substrate accessibility to the enzyme and thereby properly subverting cholesterol metabolism (for testimonials see Kaushal et al. 2003; Undas et al. 2004; Liao and Laufs 2004). These efficient drugs have, however, the spectrum of activities significantly broader than could be explained only by decrease in cholesterol synthesis. They constituteAddress correspondence to J ef Dulak, PhD, DSc, Division of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krak , Poland. E-mail: [email protected]. Supported by Pfizer, Poland, PBZ-KBN-107/P04/2004 and by the Polish-Austrian Collaborative Grant (17/2002). Dr Jozkowicz is an International Senior Study Fellow of Wellcome Trust.Dulak et al.Pagethe pleiotropic effects, which have already been demonstrated to influence the production.