A, Ottawa, Canada; bAmsterdam UMC, University of Amsterdam, Department of Biomedical Engineering and Physics, Amsterdam, NetherlandsIntroduction: Various research have shown that plantderived nanoparticles (NPs) taken up by the intestinal cells impact intestinal function. Food-derived NP is recognized to facilitate delivery of proteins, nucleic acids such as microRNA (miRNA) and other massive molecules to intestinal tissues. Therefore, such large molecules may possibly affect gastrointestinal functions by means of NPs. Accordingly, we investigated the effect of applederived NP to intestinal transporters via containing cargos. Solutions: NP was prepared by ultracentrifugation. Lipid membrane of NP and apple-derived nucleic acid had been labelled by fluorescents to examine uptake in αLβ2 Storage & Stability Caco-2 cells making use of microscope. Expressions of mRNA and protein of transporters in Caco-2 cellsIntroduction: Nanoscale flow cytometry (NFC) is often a promising tool for phenotypic evaluation of individual modest particles such as extracellular vesicles (EVs) and viruses that happen to be αvβ8 web smaller sized than 500 nm in diameter. However, given that quite a few small EVs are at the moment at the limit of detection for commercial flow cytometers, productive detection of EVs calls for optimization of each sample preparation and instrument settings. These optimizations demand reference particles reflecting size, refractive index (RI), and fluorescence emission intensity of the labelled EVs of interest. Murine leukaemia virus (MLV) is a retrovirus 114 nm in diameter as measured by cryo-EM, with an estimated RI of 1.5. Here we showcase the monodispersed nature of those viruses and demonstrate their use as fluorescence reference particles for NFC.ISEV2019 ABSTRACT BOOKMethods: We engineered MLVs to express its envelope glycoprotein fused to green fluorescent proteins (eGFP and sfGFP) around the viral surface. MLVs have been characterized by NFC and by nanoparticle tracking evaluation. For the reason that MLVs are monodispersed, we combined scatter intensities and hydrodynamic diameter to acquire the successful RI by solving the inverse light scattering dilemma utilizing Mie theory. Outcomes: We measured an antigen density of 300 MESF of GFP per virion. Furthermore, we located that antibody labelling of this virus-associated antigen with distinctive fluorophore conjugates (PE, BV421 and AF647) modulates each scatter intensities and hydrodynamic diameter with the labelled virus. With regard to the hydrodynamic diameter, we show that the effectiveRI in the viruses might be tuned by using unique fluorophores. Summary/Conclusion: MLVs are equivalent to small EVs in size with equivalent surface location and comparable capacity for antigen expression. As opposed to synthetic beads, MLVs can be genetically engineered to express protein antigens of decision in biologically relevant and constant levels to act as internal constructive controls for phenotypic studies of EV surface marker expression. Furthermore, MLVs are monodisperse and have tuneable RI. Collectively, these properties help that MLVs are strong candidates as fluorescence reference particles for NFC. Funding: Natural Sciences and Engineering Investigation Council of Canada (NSERC)JOURNAL OF EXTRACELLULAR VESICLESPF07: Biogenesis II Chairs: Mathilde Mathieu; Hang Hubert Yin Location: Level three, Hall A 15:306:PF07.Proteomic profiling of outer membrane vesicles derived from MicA, a little RNA from Escherichia coli So Hee Leea, Yeong-Jun Parkb and Kwang-sun Kima Pusan National University, Busan, Republic of Korea; bPusan National Unive.