Non-small cell lung cancer Win Lwin Thuya1; Ross Soo1; Nicholas Syn1; Tiannan Guo2; Esther Sok Hwee. Cheow1; Ting Ting Wang1; Li Ren Kong1; Amelia Lau1; Richard Weijie Ong3; The Hung Huynh3; Andrea Li Ann Wong1; Henry Yang1; Paul Chi Lui Ho4; Newman Siu Kwan Sze2; Lingzhi Wang1; Boon Cher Goh1 Cancer Science Institute of Singapore, National Caspase-8 Proteins Purity & Documentation University of Singapore, Singapore, Singapore; 2School of Biological Sciences, Nanyang Technological University, Singapore, Singapore; 3National Cancer Centre, Singapore, Singapore, Singapore; 4Department of Pharmacy, National University of Singapore, Singapore, SingaporePT05.Identification of androgen-dependent glycosylations on the surface of extracellular vesicles derived from prostate cancer cell lines Md Khirul Islam1; Parvez Syed1; Jason P. Webber2; Guido W. URM1 Proteins Synonyms Jenster3; Kim Pettersson1; Urpo Lamminm i1; Janne Leivo1 University of Turku, Turku, Finland; 2Tissue Microenvironment Group, Division of Cancer and Genetics, College of Medicine, Cardiff University, Cardiff, Uk; 3Erasmus Healthcare Center, Rotterdam, The NetherlandsBackground: Adjustments in glycans are popular in cancer and play important part in identification of surface tumour markers. Majority ofBackground: The discovery of biofluid-based biomarkers is urgently required to improve early detection of lung cancer. Exosome-derived proteins are helpful resources in biomarker identification. Approaches: Proteomic analysis of 1 typical fibroblast and 3 NSCLC cell-derived exosomes was conducted. Exosomes were isolated by ultracentrifugation and characterized by western blot, transmission electron microscopy and Zetasizer. Human plasma and tissues samples had been employed for validation of FAM3C as a novel lung cancer in vivo biomarker. Written informed consent was obtained from all participants. Outcomes: FAM3C was among the top rated 15 possible proteins hugely expressed in cancer cell exosomes and selected for additional validation. In functional study, overexpression of FAM3C significantly stimulated the epithelial-mesenchymal transition (EMT), migration, invasion, proliferation and colony formation of lung cancer cells when knockdown of FAM3C showed opposite effects. Additional analysis showed that exosomes could serve as messengers in intercellular communication to promoteISEV 2018 abstract bookmetastasis in lung cancer cells. Injection of overexpressed FAM3C cells through the tail vein promoted lung metastasis in mouse models. The IHC staining indicated that FAM3C expression in lung cancer specimens was considerably improved in comparison with those in tumour adjacent and regular lung tissues. Additionally, granular FAM3C staining was drastically associated with enhanced lung cancer specific survival in squamous cell carcinoma sufferers. ELISA assay revealed that plasma exosome FAM3C was significantly elevated in NSCLC individuals (n = 78) when compared with healthful controls (n = 78) (p 0.0001) with an AUC of 0.831, a sensitivity of 0.756 as well as a specificity of 0.744. Summary/conclusion: These findings demonstrate that exosomederived FAM3C is actually a prospective biomarker which predicts lung cancer metastasis, and additional large-scale clinical studies are warranted. Funding: This investigation is supported by the National Investigation Foundation Singapore and the Singapore Ministry of Education under its Research Centers of Excellence initiative.PT05.Exploiting lipidomics to unravel novel biomarkers for pancreatic cancer Aikaterini Emmanouilidi1; Peter J. Meikle2; Dino Paladin1; Marco FalascaSchool of.