G that about 80.4 of the total amount of silver was accumulated within the biomass. The SEM pictures and EDAX spectrum obtained for the AgNPs-Spirulina biomass are presented in Figure 2.Spirulina biomass containing AgNPs was analyzed so as to determine silver contentabFigure two. AgNPs-Spirulina (protein DNQX disodium salt custom synthesis fraction) a. SEM; b. EDAX. Figure two. AgNPs-Spirulina (protein fraction) a. SEM; b. EDAX.3.3. Biological Parameters of Animals and Organs immediately after AgNPs and AgNPsSp Administration During the experiment, the rats in all groups gained weight, and no visible differences had been observed involving them. Thus, males elevated their body mass by 5055 more than 28 days, and females improved their physique mass by 526 . No variations within the macroscopic and histological appearances of the collected organs had been detected. ThereFigure 2. AgNPs-Spirulina (protein fraction) a. SEM; b. EDAX.Nanomaterials 2021, 11,6 of 14 three.3. Biological Parameters of Animals and Organs right after AgNPs and AgNPsSp TMPyP4 References AdministratiDuring the experiment, the rats in all groups gained weight, and no vi differences have been observed amongst them. Thus, males elevated their physique mass by three.three. Biological Parameters of Animals and Organs just after AgNPs and AgNPsSp Administration 55 over 28 days, and females improved their physique mass by 526 . No differenc Through the the macroscopic experiment, the rats appearances of your collected organs differences and histological in all groups gained weight, and no visible were detected. T have been observed involving them. Hence, males enhanced their body mass by 505 over 28 have been also no considerable differencesmass by 526 . No variations inside the(Table S2). days, and females improved their physique in terms of the mass of organs macroscopicand histological appearances in the collected organs had been detected. There were also no substantial differences with regards to the mass of organs (Table S2). three.four. Content material of Silver in Animal Organs following Administration of AgNPs and After aPeof Clearance3.4. Content of Silver in Animal Organs Following Administration of AgNPs and just after a Period of Clearance The silver content material determined within the organs of the laboratory animalsis showFigure The silver content material from Figure 3A, organs of thedetected in all animal organs, exce three. As observed determined inside the silver was laboratory animals is shown in theFigure three. As observed from Figure in which was detected in all animal organs, except ovarian and testicular glands 3A, silver only functionalized nanoparticles accumul within the ovarian and testicular glands in which only functionalized nanoparticles accuThe highestThe highest content material of silver was determined in the brain, liver,and kidney samp mulated. content of silver was determined inside the brain, liver, and kidney 0.145.150 /g. samples–0.145.150 /g.Figure three. The content of silver in rats’ organs: (A) animals administrated with AgNPs and AgNPs-Spirulina for 28 days, measured promptly after the finish from the experiment; (B) animals administrated with AgNPs for 28 days with measurements soon after a clearance period; (C) animals administrated with AgNPs-Spirulina for 28 days after a clearance period. 28 CET–clearance expiry time of 28 days. indicates p 0.005 for the distinction amongst neighboring groups.The administration of AgNP-Spirulina made results unique from those obtained for animals administrated with unmodified nanoparticles. The highest content material of silver was determined in the brain and kidneys, while the amounts of silver accumulated in the splee.