G: This function was supported by grants in the Japan Society for the Promotion of Science (20K22969) to M.M.C.-R. (80272152; 18H04061) and to C.C. Institutional Critique Board Statement: All experimental protocols were authorized by the University of Tsukuba Safety Committee for Recombinant DNA Experiments (Code: 170110 from 1 December 2017) in which the policy with the Animal Care and Use Committee in University of Tsukuba is included. Informed Consent Statement: Not applicable. Information Availability Statement: All information utilised in this study are obtainable in the corresponding author upon affordable request. Acknowledgments: We thank all members of the Japan Newt Investigation Community (JNRC) and the Newtic Organ Regeneration Forum for their valuable comments and discussion, and also the citizens of Toride City for assisting us with all the daily maintenance from the newts. Conflicts of Interest: The authors declare no conflict of interest.12-Hydroxydodecanoic acid custom synthesis Biomedicines 2021, 9,17 of
biomedicinesArticlePhoenixin as a brand new Target in the Improvement of Strategies for Endometriosis Butenafine Autophagy Diagnosis and TreatmentKarolina Iwona Kulinska 1, , Miroslaw Andrusiewicz 1, , Anna Dera-Szymanowska two, , Maria Billert three , Marek Skrzypski 3 , Krzysztof Szymanowski 2,4 , Ewa Nowak-Markwitz five , Malgorzata Kotwicka 1 and Maria Wolun-Cholewa 1, Citation: Kulinska, K.I.; Andrusiewicz, M.; Dera-Szymanowska, A.; Billert, M.; Skrzypski, M.; Szymanowski, K.; Nowak-Markwitz, E.; Kotwicka, M.; Wolun-Cholewa, M. Phoenixin as a brand new Target inside the Improvement of Tactics for Endometriosis Diagnosis and Therapy. Biomedicines 2021, 9, 1427. https://doi.org/10.3390/ biomedicines9101427 Academic Editor: Nikolaos Machairiotis Received: 30 August 2021 Accepted: 5 October 2021 Published: 9 OctoberChair and Division of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806 Poznan, Poland; [email protected] (M.A.); [email protected] (M.K.) Clinic of Perinatology and Gynaecology, Poznan University of Health-related Sciences, Polna 33, 60-535 Poznan, Poland; [email protected] (A.D.-S.); [email protected] (K.S.) Department of Animal Physiology, Biochemistry and Biostructure, Poznan University of Life Sciences, Wolynska 35, 60-637 Poznan, Poland; [email protected] (M.B.); [email protected] (M.S.) Chair and Division of Health-related Education, Poznan University of Health-related Sciences, Rokietnicka 7, 60-806 Poznan, Poland Gynecologic Oncology Division, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland; [email protected] Correspondence: [email protected] (K.I.K.); [email protected] (M.W.-C.) These authors contribute equally to this perform.Abstract: Little integral membrane protein 20/phoenixin (SMIM20/PNX) and its receptor GPR173 (G Protein-Coupled Receptor 173) play a part inside the regulation with the hypothalamic ituitary onadal axis (HPG). The aim of the study was to identify PNX, FSH, LH, and 17-estradiol association in females with endometriosis, plus the expression of SMIM20/PNX signaling via GPR173. Serum PNX, FSH, LH, and 17-estradiol concentrations have been measured by enzyme and electrochemiluminescence immunoassay. SMIM20/PNX and GPR173 expression inside the eutopic and ectopic endometrium was assessed by qPCR and immunohistochemistry. Decreased PNX level, elevated LH/FSH ratio and elevated 17-estradiol concentration have been discovered in individuals with endometriosis. No variations in SMIM20 expression had been observed amongst the studied endometria. GPR173 expression was reduced in ecto.