N the intracellular chloride calibration profile, perfusate and endosomal chloride concentrations were equalized by incubating the previously fixed cells in the appropriate chloride clamping buffer containing a precise concentration of…
Lofen). Statistical evaluation was performed with two sample t-test p0.05, p0.01, ns: p=0.5 (C) and p=0.63 (D). DOI: ten.7554/eLife.26147.Badheka et al. eLife 2017;6:e26147. DOI: ten.7554/eLife.13 ofResearch articleNeurosciencewhich is constant with…
Osomal chloride concentrations to 104 and 106 mM respectively, indicating that Clensor was capable of measuring pharmacologically induced lysosomal chloride changes, if any, in these cells. In Gaucher's cell culture…
Cs, http://orcid.org/0000-0003-3580-2575 Ethics Animal experimentation: Animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Rutgers New Jersey Health-related College. Animals were handled based on the…
N the intracellular chloride calibration profile, perfusate and endosomal chloride concentrations had been equalized by incubating the previously fixed cells in the acceptable chloride clamping buffer containing a particular concentration…
Ternalized by the coelomocytes resulting in GFP labeling of your coelomocytes (Fares and Greenwald, 2001). Following 1 hr, both devices quantitatively colocalize with GFP indicating that they especially mark endosomes…
E I-switch sample was diluted to 500 nM working with 1X Medium 1. Briefly, worms were incubated at 22 for 1 hr post microinjection after which immersed in clamping buffers…
N diverse RNAi background. DOI: 10.7554/eLife.28862.Chakraborty et al. eLife 2017;six:e28862. DOI: 10.7554/eLife.7 ofResearch articleCell BiologyClensor respectively, in each 461054-93-3 supplier genetic background at 60 min post injection (Figure 3a and…
Re supplement two. PI(three,4)P2/PIP3 generation is diminshed by PI3K inhibitor wortmannin. DOI: https://doi.org/10.7554/eLife.38869.010 Figure supplement three. TRPV1 co-expression will not alter PI3K expression. DOI: https://doi.org/10.7554/eLife.38869.011 Figure supplement 3--source information 1.…
Clofen (25 mM) were diluted 20x inside a sterile car answer of 10 PEG-200 (SigmaAldrich), 2 Tween-80 (Amresco) in 0.9 NaCl. Hind paw subcutaneous dorsal injection of 10 mL was…