I [27]. Prior to the experiment, participants were verbally instructed that the experimenter
I [27]. Just before the experiment, participants were verbally instructed that the experimenter would draw letters on their forehead or their neck (around the back of your head beneath the hairline) by using a cotton bud (Fig 4A). Participants have been informed that only certainly one of those letters , b, p, q, n, v, w and o ould be drawn on their skin, and they had been instructed to report as spontaneously as you can the letter they skilled (Fig 4B). The experimenter traced the letters by PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/29046637 making use of a cotton bud in a single continuous motion on the skin. To enhance the difficulty of your task and to not cue participants to choose a strategy depending on the direction in the writing, all letters have been drawn using the motion starting from 1 or the other finish of each and every letter, so that letters have been traced as outlined by a canonical or noncanonical direction of writing. As a result, participants first had to make a representation on the worldwide shape of your letter before providing an answer, because the path of the writing was uninformative. Participants have been comfortably seated on a chair and have been instructed to close their eyes throughout the recording session. In 1 session, the experimenter sat in front from the participant and drew the letters around the central a part of the participant’s forehead. Inside the other session, the experimenter sat in the back with the participant and drew the letters on the participant’s neck. Every session comprised 48 trials, such as 32 presentations of ambiguous letters (8 presentations of d, b, p and q) and six presentations of nonambiguous 
letters (4 presentations of n, v, w and o). Letters with their path of drawing have been Ro 67-7476 site presented on a computer system screen towards the experimenter in a randomized order by utilizing PsychoPy2 (v.82.0) [48]. The experimenter utilized aFig four. Procedures for the graphaesthesia activity (Experiment two). (A) The experimenter drew letters around the participants’ forehead and neck by utilizing a cotton bud though participants kept their eyes closed. (B) Letters included ambiguous, nonsymmetrical letters (b, d, p, q) and nonambiguous, symmetrical letters (w, n, o, v) that have been all drawn inside the canonical path of writing or in the reverse path, starting from the opposite finish in the letter. All letters have been drawn by using a single and continuous hand motion. doi:0.37journal.pone.070488.gPLOS A single DOI:0.37journal.pone.070488 January 20,0 Anchoring the Self to the Physique in Bilateral Vestibular Losskeyboard to manually enter participants’ verbal responses, which have been saved by utilizing PsychoPy and processed offline. Information evaluation. To measure the degree of anchoring the self towards the physique, we calculated the proportion of ambiguous letters (d, b, p and q) that were skilled from a firstperson perspective (e.g when participants reported the letter “q” right after the experimenter drew the letter “p” on their skin) [23]. The capability to appropriately represent the pattern of letters drawn on the skin was calculated as the proportion of right identification of symmetrical letters (n, v, w and o). Information had been analyzed by using repeatedmeasures ANOVAs using the Web-site of stimulation (forehead vs neck) as a withinsubject factor and with all the Group (BVF individuals vs controls) and Gender (female vs male) as betweensubject elements.ResultsAnalysis of your proportion of ambiguous letters experienced from a firstperson perspective revealed a important main impact on the Web-site of stimulation (F,42 68.96, p0.00, 2p 0.62). Fig 5 shows that letters had been far more normally seasoned from a first.