Rs from the Chloroflexi,Firmicutes,and Proteobacteria (L fler and Edwards Maphosa et al. Hug et al. Dehalobacter spp. dechlorinate chlorinated ethenes (Wild et al. Holliger et al,chlorinated ethanes (Sun et al. Grostern and Edwards,a,b; Grostern et al,chlorobenzenes (Nelson et al,and other halogenated compounds (Schlotelburg et al. van Doesburg et al. Yoshida et al a,b; Wang et al. The genus Dehalobacter belongs for the phylum Firmicutes and is phylogenetically closely associated towards the genus Desulfitobacterium (Villemur et al. But in contrast to Desulfitobacterium spp that are recognized for their metabolic versatility,most members from the Dehalobacter group are specialized for organohalide respiration,a way of life akin to Dehalococcoides or Dehalogenimonas in the Choroflexi (Siddaramappa et al. Even so,two Dehalobacter populations capable of fermentation of dichloromethane were lately described (JusticiaLeon et al. Lee et al,indicating members on the Dehalobacter genus is often far more versatile than Dehalococcoides. Previously,we reported the de novo assembly of the very first two complete Dehalobacter genomes from metagenomic information derived from an enrichment culture named ACT,that dechlorinates ,,triJW74 cost chloroethane (,TCA) to chloroethane (CA) through ,dichloroethane (DCA),as well as dechlorinates chloroform (CF) to dichloromethane (DCM; Tang et al. Within this culture,the twostep dechlorination of ,,TCA to ,DCA followed by ,DCA to CA is catalyzed by two distinct Dehalobacter populations. The very first population dechlorinates ,,TCA to ,DCA and CF to DCM applying a specific reductive dehalogenase (RDase),CfrA,when the second population dechlorinates ,DCA to CA working with a distinct RDase,DcrA. We’ve now isolated strain CF and strain DCA corresponding to the two assembled genomes (Tang. We also previously identified that the two active RDases CfrA and DcrA belonging to each strain share . amino acid identity and . nucleotide identity,even though a single dechlorinates CF and ,,TCA and also the other dechlorinates ,DCA,with no crossreactivity. These had been the only two RDase genes discovered expressed within the ACTculture grown with ,,TCA as electron acceptor (Tang and Edwards. Within this paper,we report the detailed evaluation of those two Dehalobacter genomes in comparison with 3 other Dehalobacter genomes: the comprehensive genome of Dehalobacter restrictus strain PERK (Kruse et al,the draft genome of Dehalobacter sp. strain E (Maphosa et al plus the draft genome of Dehalobacter sp. strain UNSWDHB (Deshpande et al. In addition,we examine Dehalobacter genomes to those of Desulfitobacterium hafniense Y (Nonaka et al and Dehalococcoides mccartyi strain (Seshadri et al as contrasting organohalide respiring bacteria. The physiological characterization of strains CF and DCA is ongoing and also the complementary experimental data in Tang’s PhD thesis (Tang,will likely be reported separately.Final results AND DISCUSSION Basic Genome FeaturesThe three complete Dehalobacter genomes of strains CF,DCA and PERK are comparable in size Mb) and GC content material ( and comprise coding sequences (CDS; Table. The draft genome of strain E was sequenced with pyrosequencing with coverage and consists of contigs (Maphosa et al. The draft genome of strain UNSWDHB was Illumina pairedend sequenced with coverage and consists of contigs (Deshpande et al. Since the draft genomes of strains E and UNSWDHB were assembled from shortread sequencing data,extended interspersed repeats,such as multicopy transposable elements (TEs) and ribosomal RNA (rRNA) genes,are PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20507800 not nicely rep.