The gene substitution vectors pDAPF1 (HPH), pDAPF2 (HPH), pDAPF3 (HPH), pDAPF6 (HPH), pDAPF9 (HPH) and pDAPF11 (NAT) have been developed using the yeast recombinational program
For making deletion mutants, the putative flanking locations of the desired gene were AKT inhibitor 2 distributor amplified utilizing the primer pair ``gene''-3R and ``gene''-3F for the 39 flank and…