A densitometric analysis of the respective bands exposed a cleavage effectiveness of around 50% for the wild kind peptide
The optimum recognition internet site for TEV is Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S)) and cleavage happens amongst the Gln and Gly/Ser residues . DENV NS4A begins with serine and thus TEV cleavage enables…