In control groups, the embryos ended up injected with only Cy3-DNA fragments or Cy3-DNA fragments provided in the native I-SceI endonuclease digestive response technique as that for ISceI-mediated transgenesis in fish
The injected embryos were being cultured and noticed below LSCM at sixteen and 24 h submit activation.At 16 h submit activation, in the embryos co-injected with NLS-I-SceI mRNAs and Cy3-DNAs,…