Incubation with 2 times as substantially lysates yielded very similar effects, indicating that maximal EphA3 binding to the antibody immobilized in the wells was realized

Coimmunoprecipitation experiments employing HEK293 cells coexpressing mCherry-ephrin-A3 with EphA3 N or the N G518L mutant uncovered far more ephrin-A3 related with the mutant (Determine 4A). Measurement of ephrin-A5 AP binding…